Analytical methods for Challenge4

Sample: 10 microM in methanol/water (50:50)

Instrument: LTQ Orbitap Velos (Thermofisher Scientific)
Resolution: 30,000
Ion source: nano-ESI, positive ion mode
Spray voltage: 2.2 kV 
Sample Introduction: Infusion at the flow rate 500 nanoL/min
CID condition: 22 V
Precision on m/z: 3 ppm or better
Scan range: 
      FTMS + p NSI Full MS [50.00-400.00]
      FTMS + p NSI Full MSMS 211.11@hcd22.00 [50.00-220.00]