LC-MS/MS analytical methods for Challenge1 Sample: The compound is a secondary metabolite isolated from Solanaceae plants. It contains one or more aromatic structures. Mass spectrometer: LCMS-IT-TOF (Shimadzu) Type of mass spectrometer: Iontrap-TOF Ion source: ESI, positive ion mode Nebulizer gas flow: 1.5 L/min CDL temperature: 200 C Heat block temperature: 200 C Ion accumulation: 100 msec Collision energy: 120% (device-dependent value) Collision gas: 100% (device-dependent value) Precursor selection width: 3 Da Mass accuracy: 10 ppm or better LC column: Agilent Poroshell 120 EC-C18 (2.1 x 75 mm) Solvents: H2O (0.1% formate)/acetonitrile (0.1% formate) Flow rate: 0.2 mL/min Gradient: 10-70% acetonitrile for 8 min